Journal: Cells

Article Title: Development of a Custom Fluid Flow Chamber for Investigating the Effects of Shear Stress on Periodontal Ligament Cells

doi: 10.3390/cells13211751

Figure Lengend Snippet: Western blot analysis of PTGS2/COX2 and RUNX2 proteins after 1 h fluid shear stress. FSS induced the expression of PTGS2 but not RUNX2. Lysates from donor 1 ( A ) and donor 2 ( B ) from 1 h FSS, the corresponding control (Ctrl), and the positive controls for GAPDH (HeLa), COX2, and RUNX2 (both expressed in baculovirus-insect cells) were separated by PAGE on a 14% SDS gel and transferred onto a PVDF membrane.

Article Snippet: Shortly, after 1 h FSS, the slides were transferred to a petri dish (628160, CELLSTAR ® , Greiner Bio-One GmbH, Frickenhausen, Germany) and washed twice with DPBS (D8537; Merck/Sigma-Aldrich, Darmstadt, Germany).

Techniques: Western Blot, Shear, Expressing, Control, SDS-Gel, Membrane

Journal: Cornea

Article Title: Evaluating Moisture Migration in Schirmer Test Strips: Exploring Brand-Specific Variations and Introducing Calibration and Conversion Methods

doi: 10.1097/ICO.0000000000003430

Figure Lengend Snippet: Physical Characteristics (Mean and SD) of Five Different Brands of Commercially Available Schirmer Strips

Article Snippet: Each dry Schirmer strip was weighed on the plexiglass surface of a cell culture dish lid (Cell star 628 160; Greiner Bio-One GmbH, Frickenhausen, Germany) inside the calibrated scale, and its dry weight was determined.

Techniques: Stripping Membranes, Sterility

Journal: Cornea

Article Title: Evaluating Moisture Migration in Schirmer Test Strips: Exploring Brand-Specific Variations and Introducing Calibration and Conversion Methods

doi: 10.1097/ICO.0000000000003430

Figure Lengend Snippet: A, Photograph of 5 different brands of Schirmer strips used to compare moisture migration. Parallel alignment of dry strips shows differences in shape, length, width, and millimeter scales between manufacturers. (1) Haag-Streit, (2) Contacare Ophthalmics and diagnostics, (3) TearFlo, (4) MIPL/A6, and (5) EagleVision/Corza Medical. B, Photograph of the different brands 5 minutes after application of 5 μL of human tears at the tip of the strips. The red lines show the wide range of moisture migration lines observed for the same volume of tears applied. All moisture migration values were read from imprinted scale except for Haag-Streit which was measured with digital calipers: (1) Haag-Streit (unmarked strip) = 5 mm, (2) Contacare = 9 mm, (3) MIPL = 9 mm, (4) TearFlo = 8 mm, and (5) EagleVision = 10.5 mm.

Article Snippet: Each dry Schirmer strip was weighed on the plexiglass surface of a cell culture dish lid (Cell star 628 160; Greiner Bio-One GmbH, Frickenhausen, Germany) inside the calibrated scale, and its dry weight was determined.

Techniques: Migration, Stripping Membranes

Journal: Cornea

Article Title: Evaluating Moisture Migration in Schirmer Test Strips: Exploring Brand-Specific Variations and Introducing Calibration and Conversion Methods

doi: 10.1097/ICO.0000000000003430

Figure Lengend Snippet: Measured moisture migration for different tear volumes. Shown are replicate data points (triangles, circles, and crosses) for each experiment performed in triplicate for the different brand Schirmer strips, indicated by different colors. For each brand, the best-fit linear regression is shown as colored solid line, with slopes and offsets for the different brands listed in Table , along with the rmsd of the data points to the straight line. The overall consensus regression of all data points is shown as dotted black line, has a slope of 1.43 mm/μL with an offset of −0.38 mm, and fits with an overall rmsd of 2.89 mm.

Article Snippet: Each dry Schirmer strip was weighed on the plexiglass surface of a cell culture dish lid (Cell star 628 160; Greiner Bio-One GmbH, Frickenhausen, Germany) inside the calibrated scale, and its dry weight was determined.

Techniques: Migration

Journal: Cornea

Article Title: Evaluating Moisture Migration in Schirmer Test Strips: Exploring Brand-Specific Variations and Introducing Calibration and Conversion Methods

doi: 10.1097/ICO.0000000000003430

Figure Lengend Snippet: Conversion Table for Millimeters of Moisture Migration of Tears to Microliter Volume in Different Brands of Schirmer Strips

Article Snippet: Each dry Schirmer strip was weighed on the plexiglass surface of a cell culture dish lid (Cell star 628 160; Greiner Bio-One GmbH, Frickenhausen, Germany) inside the calibrated scale, and its dry weight was determined.

Techniques: Migration